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dna ligation kit mighty mix  (TaKaRa)


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    Structured Review

    TaKaRa dna ligation kit mighty mix
    Dna Ligation Kit Mighty Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 699 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+ligation+mix+mighty+mix/DNA+Ligation+Kit%2C+Mighty+Mix/bio_rxiv__64898__2026__09__30__755837-153-8-13
    Average 96 stars, based on 699 article reviews
    dna ligation kit mighty mix - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector.
    Article Snippet: A new plasmid pCASE1 was isolated from Gram-positive Corynebacterium casei JCM 12072.. It comprised a 2.4-kb nucleotide sequence with three ORFs, two of which were indispensable for autonomous replication in Corynebacterium glutamicum.. Homology search identified these two ORFs as repA and repB, areas coding proteins involved in plasmid replication. repA sequence showed high similarity to theta-replicating Escherichia coli ColE2-P9 plasmids and even higher similarity to plasmids derived from Gram-positive bacteria belonging to a subfamily of this ColE2-P9 group.

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    DNA Ligation:

    Article Title: Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector.
    Article Snippet: A new plasmid pCASE1 was isolated from Gram-positive Corynebacterium casei JCM 12072.. It comprised a 2.4-kb nucleotide sequence with three ORFs, two of which were indispensable for autonomous replication in Corynebacterium glutamicum.. Homology search identified these two ORFs as repA and repB, areas coding proteins involved in plasmid replication. repA sequence showed high similarity to theta-replicating Escherichia coli ColE2-P9 plasmids and even higher similarity to plasmids derived from Gram-positive bacteria belonging to a subfamily of this ColE2-P9 group.

    Article Title: TBP regulates transposable element expression in early mouse embryos
    Article Snippet: AccuPrime Pfx DNA polymerase , Invitrogen , 10472482. .. DNA ligation mix “Mighty Mix” , Takara , 6023. .. GoScript Reverse Transcription System , Promega , A5000.

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    TA Cloning:

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.

    Incubation:

    Article Title: A Water-Saving Drought Survival Phenotype in a Wheat TILLING Mutant Involves Survival-Biased Metabolic and Phosphorylation Reprogramming.
    Article Snippet: .. For TA cloning, 0.5 μL of T‐Vector pMD20 (Takara Bio Inc., Shiga, Japan; Cat. No. 3270), 0.5 μL of PCR product, and 1.5 μL of ultrapure water were mixed in a 1.5 mL tube, followed by the addition of 2.5 μL of DNA Ligation Mix (Takara Bio; Cat. No. 6023) and incubation at 16°C for 30 min. .. The entire ligation mixture was transformed into 50 μL of DH5α competent cells (Takara Bio, Cat. No. 9057) and spread on LB agar plates containing 100 μg/mL ampicillin.



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